• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to the use of microorganisms as stimulants of plant growth and for the biological control of bacteria, insects, fungi and phytopathogenic nematodes. In particular, the invention relates to the use of microorganisms of the genus Bacillus, in particular the Bacillus methylotrophicus species, as well as relating to the cultures thereof, compositions containing these bacteria, different cultivation methods, and the products comprising same, as stimulants of plant growth and for the biological control of bacteria, insects, fungi and phytopathogenic nematodes.
    公开号:ES2639375A1
    申请号:ES201790002
    申请日:2015-07-31
    公开日:2017-10-26
    发明作者:María Victoria BÉJAR LUQUE；Inmaculada Llamas Company；Cristina RUÍZ GARCÍA；Emilia Quesada Arroquia
    申请人:Universidad de Granada；
    IPC主号:
    专利说明:

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    The strain XT1 (deposit number CECT8661), object of the invention was isolated in 1999 from a sample of the rhizosphere of a soil near the Capacete lagoon, located in Fuente de Piedra, Málaga (Spain). The strain XT2 (deposit number 5 CECT8662) was isolated in 2010 in a soil near the mouth of the Velez River (Malaga, Spain). The medium used was the MY medium (Moraine and Rogovin 1966) added with 7.5% of sea salts in the case of strain XT1 and the medium MY with 3% of sea salts in the case of strain XT2. Both strains were selected for their characteristics among some 5000 colonies (looking for those with the highest
    10 surfactant activity by the method of Jain et al. 1991). Example 2. Use of strains XT1, XT2 and type strain as antifungals
    The activity against fungi (table 1) was carried out by sowing strains XT1, XT2, the type strain and that of Botrybel in a small extension in PDA medium (dextrose potato
    15 agar). Next, a piece of agar of approximately 1 cm2 was placed at the opposite end with mycelium of the fungus to be tested, and after 20 days and after incubation at 25 ° C, the maximum and minimum radius of the fungus mycelium was measured to calculate the percentage of fungus growth reduction
    twenty
    Mushrooms Antifungal activity
    XT1 XT2Strain typeBotrybel
    Alternaria alternata 27 and 14 (49)24 and 19 (21)25 and 8 (68)24 and 20 (17)
    Aspergillus niger 30 and 22 (27)25 and 10 (60)ND0
    Botrytis cynerea 45 and 8 (83)45 and 2 (96)40 and 20 (50)46 and 16 (56)
    Fusarium oxysporum 30 and 28 (7)032/29 (10)0
    Phytophthora cactorum 8 and 3 (63)8 and 3 (63)ND8 and 6 (21)
    Phytophthora cinnamomi 22 and 16 (28)22 and 15 (32)16 and 12 (21)20 and 14 (30)
    Rhizopus oryzae 45 and 8 (82)50 and 12 (76)ND40 and 7 (82)
    Sclerotinia sclerotiorum 16 and 7 (56)16 and 12 (25)16 and 10 (37)ND
    Thanatephorus cucumeris 12 and 7 (42)16 and 10 (37)16 and 7 (56)ND
    Verticillium dahliae 25 and 12 (52)24 and 12 (50)25 and 14 (44)23 and 13 (44)
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    权利要求:
    Claims (1)
    [1]
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    优先权:
    申请号 | 申请日 | 专利标题
    ESP201431158|2014-07-31|
    ES201431158A|ES2561908B2|2014-07-31|2014-07-31|Use of Bacillus methylotrophicus as a plant growth stimulant and biological control medium, and strains isolated from that species|
    PCT/ES2015/070600|WO2016016508A1|2014-07-31|2015-07-31|Use of bacillus methylotrophicus as a stimulant of plant growth and biological control means, and isolates of said species|
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